The test measures a set of miRNAs by qRT PCR to classify a nodule as benign or ‘suspicious for malignancy by miRNA profiling’. The test also measures a miRNA specific to medullary carcinoma. The negative predictive value (NPV) in indeterminate nodules where all three reviewing pathologists were in agreement regarding final diagnosis is 99%; it is 91% for the entire validation set.MethodsPatients and samplesThe study was composed of three stages: (1) discovery, (2) training and (3) validation (figure 1).
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